Journal: The EMBO Journal

Article Title: HAND1 level controls the specification of multipotent cardiac and extraembryonic progenitors from human pluripotent stem cells

doi: 10.1038/s44318-025-00409-0

Figure Lengend Snippet: Reagents and tools table

Article Snippet: HES3 NKX2-5 eGFP/w hESCs (Elliott et al, ) and gene-edited derivatives were maintained on a layer of mitotically-inactivated mouse embryonic fibroblasts (MEFs) in DMEM/F12-based medium containing 20% (v/v) KnockOutTM Serum Replacement (Gibco), 100 mM non-essential amino acids (Gibco), 2 mM GlutaMAXTM (Gibco), 0.1 mM β-mercaptoethanol (Gibco) and 10 ng/ml bFGF (Miltenyi Biotech). hPSCs were passaged using TrypLE Select (ThermoFisher ScientificTM) every 3–4 days.

Techniques: Recombinant, Sequencing, CRISPR, Knock-In, Knock-Out, Membrane, Electroporation, Bicinchoninic Acid Protein Assay, Western Blot, Staining, Protease Inhibitor, cDNA Synthesis, SYBR Green Assay, Sample Prep, Software, Real-time Polymerase Chain Reaction, Imaging, Microscopy, Flow Cytometry